Table 3. Site-Specific N-glycan Analysis of EPO Expressed in CHO^WT and CHO^KO:mgat1.

		CHOKO:mgat1
EPO N-glycosite	CHOWTa	Man5-GlcNAc2-Fuc/totalb	Man5-GlcNAc2/totalb
N51	biantennary	0.48	0.52
N65	tetra-antennary	0.90	0.10
N110	tetra-antennary	0.67	0.33

^aGlycans at all three sites of EPO in CHO^WT are exclusively core-fucosylated.

^bTotal is the sum of top five glycopeptide intensities at each site by LC–MS.