Table 1.
Minimum inhibitory concentrations for isolates recovered in this study
| Sample No |
BRD Pathogen |
Culture Result | AMP | TIO | CLIN* | DANO | ENRO | FLOR | GAM | GEN* | NEO* | PEN | SPECT | SUL* | TET | TIAM* | TILD | TILM | TRIMS* | TULA | TYL* | CTET | OTET |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | M. bovis | - | - | - | - | 0.12 | 4 | 128 | - | - | 16 | - | - | - | - | 256 | 512 | - | 64 | 256 | 4 | 2 | |
| 2 | M. bovis | - | - | - | - | 0.12 | 4 | 64 | - | - | 16 | - | - | - | - | 256 | 512 | - | 8 | 32 | 8 | 4 | |
| 3 | P. multocida | Few | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 16 | 4 | 64 | 0.12 | 128 | 512 | 8.0 | 64 | 32 | 32 | 2 | 128 | 64 | - | - |
| 5 | M. bovis | - | - | - | - | 8.00 | 8 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 256 | 4 | 4 | |
| 5 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 4 | 8 | 0.12 | 32 | 256 | 0.5 | 32 | 1 | 4 | 2 | 8 | 32 | - | - |
| 6 | P. multocida | 2 + | 0.50 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 2 | 4 | 64 | 1.00 | 32 | 512 | 8.0 | 32 | 2 | 16 | 4 | 8 | 64 | - | - |
| 7 | M. bovis | - | - | - | - | 4.00 | 4 | 256 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 32 | 16 | 8 | |
| 7 | P. multocida | 1 + | 0.25 | 0.25 | 16 | 0.12 | 0.12 | 0.50 | 1 | 1 | 4 | 0.25 | 32 | 512 | 0.5 | 8 | 1 | 2 | 2 | 8 | 16 | - | - |
| 8 | M. bovis | - | - | - | - | 4.00 | 8 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 256 | 8 | 4 | |
| 8 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 2 | 8 | 0.12 | 32 | 256 | 0.5 | 16 | 1 | 4 | 2 | 8 | 32 | - | - |
| 9 | M. bovis | - | - | - | - | 4.00 | 4 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 256 | 8 | 4 | |
| 9 | P. multocida | 4 + | 0.50 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 2 | 4 | 64 | 2.00 | 32 | 512 | 4.0 | 64 | 2 | 16 | 4 | 8 | 64 | - | - |
| 10 | M. bovis | - | - | - | - | 0.12 | 2 | 32 | - | - | 16 | - | - | - | - | 128 | 256 | - | 8 | 32 | 8 | 4 | |
| 10 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 4 | 16 | 0.25 | 32 | 512 | 0.5 | 32 | 1 | 4 | 2 | 8 | 32 | - | - |
| 11 | M. bovis | - | - | - | - | 2.00 | 2 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 2 | 256 | 1 | 1 | |
| 11 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 4 | 8 | 0.12 | 32 | 256 | 0.5 | 32 | 1 | 8 | 2 | 8 | 64 | - | - |
| 12 | M. bovis | - | - | - | - | 4.00 | 2 | 512 | - | - | 16 | - | - | - | - | 256 | 128 | - | 512 | 256 | 4 | 2 | |
| 12 | P. multocida | 3 + | 4.00 | 0.25 | 32 | 0.12 | 0.12 | 0.25 | 1 | 4 | 64 | 16.0 | 32 | 512 | 4.0 | 64 | 2 | 8 | 4 | 8 | 64 | - | - |
| 13 | M. bovis | - | - | - | - | 0.50 | 4 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 64 | 4 | 2 | |
| 14 | M. bovis | - | - | - | - | 0.12 | 4 | 128 | - | - | 16 | - | - | - | - | 128 | 256 | - | 64 | 64 | 4 | 2 | |
| 14 | P. multocida | 1 + | 16.0 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 2 | 4 | 64 | 16.0 | 32 | 512 | 4.0 | 64 | 2 | 16 | 4 | 8 | 64 | - | - |
| 17 | M. haemolytica | 1 + | 0.25 | 0.25 | 8 | 0.12 | 0.12 | 1.00 | 16 | 2 | 4 | 0.25 | 32 | 512 | 0.5 | 16 | 1 | 16 | 2 | 64 | 64 | - | - |
| 18 | P. multocida | 2 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.25 | 1 | 2 | 64 | 0.50 | 32 | 512 | 4.0 | 32 | 2 | 8 | 4 | 8 | 64 | - | - |
| 19 | P. multocida | 4 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 2 | 8 | 0.25 | 16 | 256 | 0.5 | 32 | 1 | 8 | 2 | 8 | 64 | - | - |
| 20 | P. multocida | 1 + | 4.00 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 2 | 4 | 64 | 8.0 | 32 | 512 | 8.0 | 64 | 2 | 16 | 4 | 8 | 64 | - | - |
| 23 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 2 | 8 | 0.25 | 32 | 256 | 0.5 | 32 | 1 | 8 | 2 | 8 | 64 | - | - |
| 24 | M. haemolytica | 1 + | 0.25 | 0.25 | 8 | 0.12 | 0.12 | 0.50 | 1 | 1 | 4 | 0.12 | 32 | 256 | 0.5 | 16 | 1 | 8 | 2 | 8 | 64 | - | - |
| 24 | P. multocida | 1 + | 0.25 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 2 | 8 | 0.12 | 32 | 256 | 0.5 | 32 | 2 | 8 | 2 | 8 | 64 | - | - |
| 25 | M. bovis | - | - | - | - | 0.12 | 4 | 512 | - | - | 16 | - | - | - | - | 256 | 512 | - | 512 | 256 | 8 | 4 | |
| 25 | P. multocida | 1 + | 0.50 | 0.25 | 32 | 0.12 | 0.12 | 0.50 | 1 | 2 | 8 | 0.12 | 32 | 256 | 0.5 | 32 | 1 | 8 | 2 | 8 | 64 | - | - |
Samples were screened for the presence of BRD pathogens and recovered isolates were tested for sensitivity to antimicrobials via serial broth microdilution using the BOPO7F panel. MICs for each antimicrobial were compared against CLSI breakpoints (full details in Supplementary Table 2, Additional File 2. Table entries with a dash indicate an isolate was not tested against a certain antimicrobial. Antimicrobials without established breakpoints for the target organisms are indicated with an asterisk