Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 1;13:24. doi: 10.1186/s13229-022-00502-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — POGZ promotes ESC neural induction. A Morphology of day 1, 3, and 6 EBs from control and early passage Mut1 ESCs. B Time course qRT-PCR analysis of indicated genes. C Volcano plot showing the up- and down-regulated genes (fold change > 2) of day 6 EBs from control and Pogz−/− ESCs. D Heat map showing the expression of indicated neural genes. Three replicates are shown: KO for Pogz−/− ESCs, and Ctr for control ESCs. E GO analysis of DEGs of day 6 EBs from control and Pogz−/− ESCs. F Morphology of day 2, 4, 7 neurospheres from control-, Pogz−/−, and POGZ-restoring ESCs. G Morphology of day 16 neuronal cells from control-, Pogz−/−, and POGZ-restoring ESCs. White arrows pointing to the neuronal fibers. H Time course qRT-PCR analysis of indicated neural genes during control and Mut1 ESC directional differentiation toward neural progenitors. I Flow showing the percentage of PAX6-positive cells in control and Pogz−/− ESC-derived neurospheres. J IF results showing PAX6-positive cells in day 7 neural progenitors from control, Mut1, and Mut2 ESCs. Bar: 25 μm. K Quantitation of (J) by Image J. Flow and IF experiments were repeated at least two times. qRT-PCR was repeated at least three times