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Workflow of Single-Cell RNA-sequencing PDAC tissue from patients. PDAC tissue is collected from patient donors and digested into a single cell suspension of live cells. Cells are lysed, cellular mRNA captured, and cDNA libraries are generated and subjected to high-throughput sequencing. This is followed by bioinformatics analysis, including downstream feature generation and visualization of cells clusters by Uniform Manifold Approximation and Projection (UMAP).
