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. 2022 May 19;13:753416. doi: 10.3389/fendo.2022.753416

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Copyright © 2022 Huang, Zhang, Ni, Zhou, Lv, Li, Yan and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RARα directly regulated CEBPB transcription in decidualized T-HESCs. (A) In silico (JASPAR)-predicted binding site between RARα and the CEBPB upstream -2200 promoter region. (B) Results of ChIP experiment performed in decidualized T-HESCs. (C) C/EBPβ mRNA expression levels in RARα-knockdown T-HESCs absent MPA and cAMP. (D) Results of ChIP experiment performed in T-HESCs absent MPA and cAMP. (E) Result of luciferase reported assay for mutation of predicted binding site (-2,009/-1,781) in the CEBPB promoter. Chromatin was immunoprecipitated with anti- RARα. ChIP-qPCR results are normalized to the input DNA. Expression of mRNA levels are normalized to GAPDH expression. Firefly/Renilla fluorescence ratios were calculated to determine the promoter activity. All data are shown as means ± SEMs. Statistically significant differences between two groups were determined by Student’s t-test. *P < 0.05, ***P < 0.001.