TABLE 2.
Variability in size estimation and the estimated relative abundance (percentage of total fluorescence) of selected fragments that represented a range in both parametersa
| Sample and peak | Fragment size (bp)
|
Fragment relative abundanceb
|
||
|---|---|---|---|---|
| Mean | SD/CV | Mean | SD/CV | |
| CB | ||||
| Peak 1 | 542.97 | 0.19/0.03 | 25.8 | 1.4/5.5 |
| Peak 2 | 589.08 | 0.28/0.05 | 22.8 | 0.7/3.2 |
| Peak 3 | 863.57 | 1.98/0.23 | 0.6 | 0.07/12.2 |
| LM | ||||
| Peak 1 | 546.35 | 0.52/0.10 | 22.8 | 1.4/6.1 |
| Peak 2 | 758.98 | 0.28/0.04 | 4.1 | 1.0/25.0 |
| Peak 3 | 889.69 | 1.61/0.18 | 3.4 | 1.1/31.1 |
| Peak 4 | 1,147.37 | 5.21/0.45 | 3.0 | 1.2/40.4 |
| SM | ||||
| Peak 1 | 544.32 | 0.17/0.03 | 6.1 | 0.7/11.8 |
| Peak 2 | 705.31 | 0.69/0.10 | 4.1 | 0.5/12.6 |
| Peak 3 | 879.17 | 1.67/0.19 | 2.1 | 0.4/18.4 |
| Peak 4 | 889.66 | 1.80/0.20 | 2.8 | 0.9/32.4 |
| Peak 5 | 1,136.43 | 0.75/0.07 | 3.3 | 0.8/25.3 |
a
The mean and SD values were determined from three PCR replicates for each sample analyzed over six to eight independent separations on the ABI 310 (total replicates = 9 to 10 for each).
b
Calculated as the ratio between the fluorescence of each fragment (peak area) and the total fluorescence of all fragments in the profile multiplied by 100.