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. Author manuscript; available in PMC: 2022 Jun 2.
Published in final edited form as: Cell Rep. 2020 Jun 2;31(9):107710. doi: 10.1016/j.celrep.2020.107710

Figure 1. Endocannabinoids Protect against Seizures.

Figure 1.

(A) Feeding 2, 20, and 200 μg/mL AEA protects against induced seizures of eas males. n = 11–12 vials/dose. Two-way ANOVA, dose: p ≤ 0.0001, time: p ≤ 0.0001, interaction: p ≤ 0.0001. Dunnett’s multiple comparison test, #: 2 μg/mL versus 0 μg/mL, p ≤ 0.05; †: 20 μg/mL versus 0 μg/mL, p ≤ 0.05; ‡: 200 μg/mL versus 0 μg/mL, p ≤ 0.05.

(B) Doses of 20 and 200 μg/mL AEA protect against seizures in eas females. n = 12 vials/dose. ANOVA with Tukey’s post hoc analysis. ****p ≤ 0.0001.

(C) A dose of 200 μg/mL 2-AG but not 2-OG and OEA protect against seizures in eas males. n = 4–12 vials/dose. ANOVA with Dunnett’s post hoc analysis. ****p ≤ 0.0001.

(D–E) A dose of 200 μg/mL AEA and 2-AG protect against seizures in tko25t flies (n = 4–12 vials/group) (D) and AEA also works in bss1 flies (n = 12–17 vials/group) (E). ANOVA with Dunnett’s post hoc analysis. **p ≤ 0.01, ****p ≤ 0.0001.

All data are presented as mean ± SEM.