KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | #A-11122; RRID: AB_221569 |
| AlexaFluor 488 goat anti-rabbit | Thermo Fisher Scientific | #A-11034; RRID: AB_2576217 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Arachidonoyl ethanolamide | Cayman Chemical | #90050 |
| 2-arachidonoylglycerol | Cayman Chemical | #62160 |
| Oleoyl ethanolamide | Cayman Chemical | #90265 |
| 2-Oleoylglycerol | Cayman Chemical | #19537 |
| Arachidonic Acid | Cayman Chemical | #90010 |
| 7Z,10Z,13Z,16Z-Docosatetraenoic Acid | Cayman Chemical | #90300 |
| URB597 | Cayman Chemical | #10046 |
| Capsazepine | Cayman Chemical | #10007518 |
| RU486 | Millipore Sigma | M8046 |
| Caffeine | Millipore Sigma | C0750 |
| AMC-arachidonoyl amide | Millipore Sigma | A6855 |
| TRIzol | Thermo Fisher Scientific | #15596026 |
| SYBR Green PCR Master Mix | Thermo Fisher Scientific | #4364346 |
| FD&C Blue | Spectrum Chemical | FD110-25GM |
| Critical Commercial Assays | ||
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | #4368814 |
| BCA Protein Assay Kit | Abcam | ab207003 |
| Deposited Data | ||
| Mendeley Dataset | This Paper | http://dx.doi.org/10.17632/vmhkt8k9p5.1 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: iso31 | Lab stock | N/A |
| D. melanogaster: easppc80f | Lab stock | Flybase ID: FBal0003489 |
| D. melanogaster: dicer;repo-GeneSwitch | Lab stock | N/A |
| D. melanogaster: nsyb-gal4 | Lab stock | N/A |
| D. melanogaster: nsyb-GeneSwitch | Lab stock | N/A |
| D. melanogaster: actin-GeneSwitch | Lab stock | N/A |
| D. melanogaster: UAS-CaLexA | Lab stock | N/A |
| D. melanogaster: parabss1 | Gift from Dr. Dan Kuebler | Flybase ID: FBal0001325 |
| D. melanogaster: tko25t | Gift from Dr. Dan Kuebler | Flybase ID: FBal0016812 |
| D. melanogaster: wtrwex | Gift from Dr. Marc Freeman | Flybase ID: FBal0246489 |
| D. melanogaster: UAS-wtrw:GFP | Gift from Dr. Marc Freeman | Ma et al., 2016 |
| D. melanogaster: y1 v1; P{TRiP.JF01904}attP2 | BDSC | BDSC ID: 25865 |
| D. melanogaster: w1118; P{GD3260}v7128 | VDRC | VDRC ID: 7128 |
| D. melanogaster: P{KK107960}VIE-260B | VDRC | VDRC ID: 100701 |
| D. melanogaster: w1118; P{GD2456}v5261 | VDRC | VDRC ID: 5261 |
| D. melanogaster: w1118; P{GD2375}v37250/TM3 | VDRC | VDRC ID: 37250 |
| D. melanogaster: w1118; P{GD2221}v39477/TM3 | VDRC | VDRC ID: 39477 |
| D. melanogaster: w1118; P{GD2221}v39478/TM3 | VDRC | VDRC ID: 39478 |
| D. melanogaster: P{KK104597}VIE-260B | VDRC | VDRC ID: 108780 |
| D. melanogaster: P{KK115661}VIE-260B | VDRC | VDRC ID: 110130 |
| D. melanogaster: w1118; P{GD2850}v42617 | VDRC | VDRC ID: 42617 |
| D. melanogaster: w1118; P{GD2850}v42620 | VDRC | VDRC ID: 42620 |
| D. melanogaster: P{KK103625}VIE-260B | VDRC | VDRC ID: 107423 |
| D. melanogaster: P{KK107656}VIE-260B | VDRC | VDRC ID: 105280 |
| D. melanogaster: w1118; P{GD372}v1366 | VDRC | VDRC ID: 1366 |
| D. melanogaster: P{KK106424}VIE-260B | VDRC | VDRC ID: 104450 |
| D. melanogaster: P{KK112299}VIE-260B | VDRC | VDRC ID: 107537 |
| D. melanogaster: w1118; P{GD1101}v6941 | VDRC | VDRC ID: 6941 |
| Recombinant DNA | ||
| Plasmid: pCFD4-U6:1_U6:3tandemgRNAs | Addgene | #49411; RRID:Addgene_49411 |
| Plasmid: pHD-DsRed-attP | Addgene | #51019; RRID:Addgene_51019 |
| Software and Algorithms | ||
| Fiji | Fiji | https://fiji.sc/ |
| Graphpad Prism | Graphpad Software | https://www.graphpad.com/ |