Figure 3.

Genetic or pharmacologic downregulation of PKA reduce cell proliferation and the expression of AQP-2, and of pro-proliferative signaling proteins and transcription factors. Western blots of regulatory and catalytic PKA subunits, and of (A) glycosylated (upper bands) and unglycosylated (lower bands) AQP-2 and pAQP-2, (B) PCNA and pro-proliferative signaling proteins and transcription factors,and (C) Gli proteins, in RiαB-expressing (left panels) and BLU2864-treated mice (right panels) and their respective controls.