Figure 4.

BLU2864 inhibits in vitro and ex vivo cystogenesis, and administration of BLU2864 by oral gavage inhibits kidney PKA activity. (A) Forskolin induced cyst formation in a three-dimensional Matrigel culture of mIMCD3 cells untreated or treated with BLU2864. (B) Forskolin induced cyst formation (left two columns) and expression of P-Ser133 CREB (right column) in metanephric organ cultures untreated or treated with BLU2864. (C) Plasma concentrations of BLU2864 3 and 15 hours after the last of five daily doses (45 mg/kg body weight [b.w.]) by oral gavage. (D) Kidney PKA activities 3 and 15 hours after the last of five daily doses of BLU2864 (45 mg/kg b.w.) by oral gavage.