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. 2022 Apr 20;13(4):10362–10372. doi: 10.1080/21655979.2022.2044734

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — miR-192 negatively modulates the expression levels of FTO. (a) miR-192 binding sites in the 3’ untranslated region of FTO as predicted by TargetScan. (b and c) Interaction of FTO with miR-192 was validated by a dual-luciferase reporter assay and RNA pull down. (d) Reverse transcription-quantitative PCR was conducted to evaluate the effects of miR-192 on FTO expression. (e) Western blot was applied to determine the protein expression of FTO. (f) Reverse transcription-quantitative PCR was used to evaluate the FTO expression after high glucose treatment. Experimental data are presented as the mean ± SD (n = 4). **P < 0.01 vs control. miR-192, microRNA-192; FTO, FTO α-ketoglutarate dependent dioxygenase.