Figure 5.

FTO regulates NLRP3 expression in a m⁶A-dependent manner. (a) Quantification of m⁶A levels in transfected ARPE-19 cells. (b) Methylated RNA immunoprecipitation was conducted to determine the enrichment of NLRP3 mRNA by utilizing m⁶A-specific antibody. (c) The mRNA stability detected using PCR. (d, e) Western blot analysis of NLRP3 expression in ARPE-19 cells transfected with FTO-expressing vector or negative control empty vector. (f) Pearson correlation analysis of the association between FTO and NLRP3. Experimental data are presented as the mean ± SD (n = 4). **P < 0.01. FTO, FTO α-ketoglutarate dependent dioxygenase; NLRP3, nucleotide-binding domain leucine-rich repeats family protein 3; m⁶A, N⁶-methyladenosine.