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. 2022 Mar 31;13(4):8950–8961. doi: 10.1080/21655979.2022.2056825

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Knockdown of TUG1 can inhibit the protective effect of EGCG on MC3T3-E1 cells. A: MTT assay-based measurement of MC3T3-E1 cell proliferation; b: Flow cytometry for the detection of MC3T3-E1 cell apoptosis; c-d: ALP staining for observing ALP activity of MC3T3-E1 cells; e: Alizarin red staining for observing the mineralization ability of MC3T3-E1 cells and quantitative analysis of the number of calcium nodules. f: qRT-PCR was applied to check the mRNA expression of Runx2, OPN, OCN, and BMP2 in MC3T3-E1 cells. **P < 0.01 vs. Control group; #P < 0.05, ##P < 0.01 vs. EGCG+ siNC group.