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. 2022 Apr 7;13(4):9483–9495. doi: 10.1080/21655979.2022.2060780

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — LMNB2 regulates cell progression. (a) Western blotting of KLF16 and LMNB2 expression in H1299 cells treated with Ctrl, KLF16, KLF16 + siLMNB2#1, and KLF16 + siLMNB2#2. (b-d) Cell viability (b), colony formation capacity (c) and cell migration (d) were measured in H1299 cells treated with Ctrl, KLF16, KLF16 + siLMNB2#1, and KLF16 + siLMNB2#2. (e) Western blotting of KLF16 and LMNB2 expression in H1975 cells transfected with siCtrl, siKLF16, and siKLF16 + LMNB2. (f-h) Cell viability (f), colony formation capacity (g) and cell migration (h) were measured in H1975 cells transfected with siCtrl, siKLF16, and siKLF16 + LMNB2. (i) Exogenously expressed Ctrl, KLF16, and KIF16+ shLMNB2 H1299 cells were injected in athymic nude mice (n = 5), and tumor formation was analyzed. Representative images and weight of xenografted tumors are shown. (j) Western blotting of KLF16 and LMNB2 expression in three xenografted tumor tissues. **P < 0.01; ***P < 0.001.