Figure 3.

GAS5 enhances NOTCH2 expression by sponging miR-29a-3p. (a) Interactions of miR-29a-3p with GAS5 and NOTCH2 were predicted by bioinformatic analyses using ENCORI (http://starbase.sysu.edu.cn/index.php) and Targetscan (http://www.targetscan.org/vert_72/). (b and c) LO2 cells were treated with control mimic or miR-29a-3p mimic. (b) MiR-29a-3p expression was examined by qPCR. (c) Luciferase activities were determined. (d) Interaction between GAS5 and miR-29a-3p was analyzed by RNA pull-down assays. (e) NOTCH2 expression in liver tissues was measured by qPCR. (f and g) GAS5 shRNA or corresponding control shRNA were transfected in LO2 cells. (f) miR-29a-3p expression was tested by qPCR. (g) NOTCH2 expression was measured by qPCR. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01.