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. 2022 Apr 12;13(4):9264–9273. doi: 10.1080/21655979.2022.2036904

Figure 4.

Figure 4.

OIP5-AS1 upregulated PD-L1 in H23 cells by targeting miR-34a. The prediction of RNAs interaction was performed by IntaRNA (a). By co-transfecting OIP5-AS1 expression vector plus negative control miRNA (NC group) or miR-34a mimic (miR-34a group) into 106 cells, dual luciferase reporter assay was also conducted (b). The luciferase activity was significantly inhibited in miR-34a group. RNA pull down was conducted (c). OIP5-AS1 cDNA could be only amplified in the precipitation pulled down by miR-34a probe but not control probe. Overexpression of OIP5-AS1, silence of OIP5-AS, overexpression of miR-34a and overexpression of PD-L1 were confirmed by RT-qPCR at 36 h post-transfection (d). Effects of OIP5-AS1 overexpression, silence and miR-34a overexpression on PD-L1 mRNA and protein expression (e). The regulatory relationship between miR-34a and OIP5-AS1 was evaluated by RT-qPCR. Overexpression of OIP5-AS1 significantly inhibited miR-34a expression (f). PD-L1 expression was detected by RT-qPCR (g). The differences between two groups were analyzed by unpaired t-test. *, p < 0.05.