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. 2022 Mar 24;13(4):8676–8688. doi: 10.1080/21655979.2022.2054501

Figure 3.

Figure 3.

MiR-21 was a target of MEG3 and IKKB was directly targeted by miR-21. a: Luciferase reporter gene assay was conducted to verify the targeting relationship of miR-21 and MEG3. ‘*’ means P < 0.05 compared with vector group. b: The regulatory effect of MEG3 on the expression of miR-21 was measured using western blot. ‘*’ means P < 0.05 compared with siRNA-NC group. ‘#’ means P < 0.05 compared with pcDNA-NC group. c: The expression of miR-21 in ICC transfected with siRNA-MEG3 and treated with TNF-α. ‘*’ means P < 0.05 compared with control group. ‘#’ means P < 0.05 compared with TNF-α group. d: The binding site between miR-21 and IKKB was predicted on the miRwalk website, and then luciferase reporter gene assay was conducted to verify the targeting relationship. ‘*’ means P < 0.05 compared with vector group. e: The regulatory effect of miR-21 on the expression of IKKB was measured using qRT-PCR and western blot. ‘*’ means P < 0.05 compared with NC group. f: The expression of IKKB mRNA and protein in ICC transfected with siRNA-MEG3 and treated with TNF-α. ‘*’ means P < 0.05 compared with control group. ‘#’ means P < 0.05 compared with TNF-α group.