Figure 5.

MiR-21/IKKB axis reversed TNF-α-induced oxidative stress, inflammation and apoptosis in ICC. ICC was transfected with miR-21 mimic and siRNA-IKKB, and then treated with TNF-α. a: MEG3 expression was detected by qRT-PCR in ICC treated with or without 20 ng/mL of TNF-α. ICC was transfected with siRNA-MEG3 and treated with 20 ng/mL of TNF-α. b: Cell viability was detected by MTT method, c-d: Flow cytometry was performed to detect cell apoptosis rate (c) and ROS level (d). e: Oxidative stress reaction markers were detected by ELISA method with a microplate reader. f: Western blot was used to determine the expression of IL-1, IL-6, Bax, Bcl2 and cleaved caspase3. ‘*’ means P < 0.05 compared with control group. ‘#’ means P < 0.05 compared with TNF-α group.