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. 2022 Mar 30;13(4):8382–8395. doi: 10.1080/21655979.2022.2036891

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Identification of BMSCs and Exo.

A. Observation of the morphology of BMSCs with an inverted microscope; B. Test of BMSCs surface markers CD44, CD73, CD34 and CD90 was via flow cytometry; C. ALP staining and Oil-Red-O staining to Examination of ALP activity and lipid after BMSCs osteogenic or adipogenic induction was via ALP staining and Oil-Red-O staining; D. Observation of Exo morphology was via TEM; E. Test of Exo-labeled proteins CD9, CD63, CD81 and TSG101 was via Western blot.