TABLE 1.

Summary of purification of the α-amylase from B. subtilis X-23^a

Column	Total activity (U)	Protein (mg)	Specific activity (U/mg)	Recovery (%)
(NH4)2SO4(ppt)	1,986	72.2	3.4	100
Q-Sepharose	1,043	25.1	41.5	52.5
Phenyl-Sepharoseb
Peak a	109	0.34	320	5.5
Peak b	485	2.16	225	24.4
Total				29.9
Superdex 75
Peak a	87.4	0.17	514	4.4
Peak b	387	1.07	362	19.5
Total				23.9

^aThe initial culture volume was 3,500 ml. 

^bThe activity was detected as two peaks in the phenyl-Sepharose elution.