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. 2022 Mar 18;13(4):8240–8254. doi: 10.1080/21655979.2022.2049471

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Astragaloside-IV (AS-IV) decreases ferroptosis in retinal pigment endothelial (RPE) cells under high glucose conditions by inhibiting expression of miR-138-5p. (a) Lipid oxidation level in cells detected by the C11-BODIPY™ 581/591 probe. (b) Total content of reactive oxygen species (ROS) detected by the CM-H2DCFDA probe. (c) Glutathione (GSH), oxidized glutathione (GSSG), and GSH:GSSG ratio analyses. (d) Cell substructure as determined by transmission electron microscopy (TEM); scale bar: 1.0 μm. (e) Cell death rate measured by flow cytometry. ** indicates p values < 0.01.