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Huaier inhibits activation of EGFR. (a, b) Western blot was used to evaluate Huaier’s inhibitory effect on EGFR. β-actin was used as internal control. (c, d) A549 and H1299 cells were treated with Huaier and stimulated with EGF (25 ng/ml) for 20 min, then the western blot was applied to measure protein levels. Histograms of results (n = 3) are presented at the bottom. Data represents mean ± SD, *p < 0.05, n = 3. Uncropped western blot gels contained in Supplementary Material 4.
