Figure 4.

CTSV knockout represses tumorigenesis in vitro. A. CTSV gene inactivation in T24 bladder cancer cell line via CRISPR/Cas9 mediated somatic cell knockout method. The expression of CTSV protein in wild-type and knockout cells was determined by immunoblotting. B. Colony formation assay performed in wild-type cells and targeted knockout CTSV cells and the quantification of their colony numbers. C. Cell growth curve assay performed in wild-type cells and targeted knockout CTSV cells. The relative cell number was measured at 450 nm by the CCK8 method on the indicated days. Error bars and mean ± SD obtained from three replicates. Statistical significance was determined by Student’s t-test or one-way ANOVA, **, p < 0.05, **, p < 0.01.