Skip to main content
. 2022 Apr 20;13(4):10180–10190. doi: 10.1080/21655979.2022.2061278

Figure 5.

Figure 5.

CTSV resulting in the NF-κB signaling pathway. A. 293 T cells were transfected using 200 ng of NF-κB luciferase reporter plasmid along with the CTSV expression plasmid in different doses or equivalent empty vector. Whole-cell extracts were used for the luciferase assay. B. NF-κB luciferase reporter plasmid was introduced into CTSV-deficient cells. Whole-cell extracts were used for the luciferase assay. C. qPCR analysis of the relative IκBα in wild-type and targeted knocked-out CTSV-T24 cells treated with TNFα (20 ng/ml, abclone, cat#: RP00001) at different times. D. RT-qPCR analysis of TNFα mRNA expression in wild-type and targeted knocked-out CTSV-T24 cells treated with TNFα (20 ng/ml, abclone, cat#: RP00001) at different times.E. Immunoblotting of the indicated protein in CTSV-overexpressed UMUC3 cells with and without PDTC. F. Colony formation assays in both wild-type cells and CTSV-overexpressed UMUC3 cells with and without PDTC with the quantification of colony numbers. Error bars and mean ± SD obtained from three replicates. Statistical significance was determined by Student’s t-test or one-way ANOVA, *, p < 0.05, **, p < 0.01, ***, p < 0.001.