Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 4;13(4):9249–9263. doi: 10.1080/21655979.2022.2059995

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — ML385 suppressed the antioxidant effects of ellagic acid (EA) and inhibited the EA-mediated increase in cartilage matrix secretion of interleukin (IL)-1β-exposed chondrocytes. C28/I2 chondrocytes were treated with Ellagic acid (EA), IL-1β (10 ng/mL), and ML385 (10 μM) for 24 h. (a) Malondialdehyde (MDA) levels were determined using the MDA assay. (b) Superoxide dismutase (SOD) activity levels were detected using the SOD activity assay. The protein expressions of SOD1 and SOD2 were assessed by (c) western blot and (d) quantification analysis. Intercellular ROS levels were determined using (e) a 2,7-dichlorofluorescin diacetate (DCFH-DA) probe and (f) quantification analysis. The protein expression of aggrecan, collagen II, and sex determining region Y-box 9 (SOX9) were determined by (g) western blot and (h) quantification analysis. (i) Secretion levels of sulfated proteoglycans were detected using Alcian blue staining. N = 3. The values are expressed as the mean ± standard deviation. *p < 0.05, **p < 0.01, and ***p < 0.001.