Figure 1.

In vivo effect of APN on BLM-induced pulmonary fibrosis. BALB/c mice were injected with BLM and different concentrations of APN. (a) The body weights of mice were recorded every three days until sacrifice. (b) Immunohistochemical analysis results of α-SMA and collagen I in lung tissues and representative images were shown. Quantitative levels of (c) α-SMA and (d) collagen I. (e) H&E and Masson staining were conducted to assess the pathological injury and fibroblastic foci. Representative images were shown. (f) Pathology index from H&E staining and (g) Ashcroft score from Masson staining were shown. (h) Protein levels of α-SMA and collagen I in the lung tissues were analyzed by western blotting. n = 5. * p < 0.05 vs. nontreated group. ns, no significant, # p < 0.05 vs. BLM-treated group.