Figure 3.
TGF-β1 mRNA expression was upregulated in ESCC tissues and positively correlated with NCK1-AS1. Luciferase reporter assay detected that TGF-β1 is related to NCK1-AS1 (a). Expression data analyzed by paired t-test showed that TGF-β1 mRNA expression was significantly upregulated in ESCC tissues compared to non-cancer tissues (b) (* p < 0.05). Linear regression showed that TGF-β1 and NCK1-AS1 were significantly and positively correlated in ESCC tissues (c) but not in adjacent non-cancer tissues (d).