Figure 1.

Rg3 reverses Cn-induced cell death in rat pancreatic acinar cells. AR42J cells were pre-incubated with 10, 20, 40 or 80 µM Rg3 for 1 h and then treated with Cn (10⁻⁸ M) for 24 h. (A) The results of CCK-8 assay revealed that the Cn-induced decrease in pancreatic acinar AR42J cell viability was reversed by pre-incubation with Rg3. (B) CCK-8 assay also demonstrated that the cell survival rate decreased following treatment with 40 µM Rg3. However, 20 µM Rg3 did not inhibit the survival rate of AR42J cells. (C) AR42J cells were pre-incubated with 20 µM Z-VAD-FMK, 1 µM Fer-1, 20 µM Nec-1, 1 µM 3-MA, 20 µM Rg3 for 1 h and then treated with Cn (10⁻⁸ M) for 24 h. (D) Flow cytometric assays demonstrated that Cn increased AR42J cell death, which was reduced following pre-incubation with Rg3. ^***P<0.001 compared with the Con group; ^#P<0.05, ^##P<0.01 and ^###P<0.001 compared with the Cn group. Cn, cerulein; Fer-1, ferrostatin-1; Nec-1, necrostatin-1; 3-MA, 3-methyladenine; Con, control.