Fig. 10. (.

A) Plot of ΔΔG^‡(k_cat) at pH 8 versus ΔG_u^o′(H₂O) for all variants. The solid line is the correlation without WT iso-1-Cytc (R = 0.96). (B) Plot of ΔΔG^‡(k_cat) at pH 8 versus ΔΔG°′_alk. The dotted line is the correlation without WT iso-1-Cytc (R = 0.57). The solid correlation line (R = 0.96) also does not include the S40T and N63T variants. ΔΔG^‡(k_cat) = −RTln(k_cat,mut/k_cat,WT). The error bars are propagated from the errors in k_cat. ΔΔG°′_alk = 2.3RT(pK_a,mut – pK_a,WT). The error bars are propagated from the errors in the pK_a for the alkaline transition.