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Plot of corrected absorbance at 695nm, A695corr, versus pH for iso-1-Cytc variants. Black lines are fits of a modified form of the Henderson-Hasselbalch equation to the data. Parameters from these fits are provided in Table 2. Data for the V57I (green circles), N63T/V57I (black circles), and S40T/V57I/N63T (red circles) are shown.
