Table 1.
Thermodynamic parameters for GdnHCl unfolding of iso-1-Cytc variants at 25 °C.
| Variant | ΔGuo′(H2O) (kcal/mol) | m (kcal/mol×M) | Cm (M) |
|---|---|---|---|
| WTa | 5.05 ± 0.30 | 4.24 ± 0.13 | 1.19 ± 0.04 |
| V57I | 4.22 ± 0.13 | 3.54 ± 0.12 | 1.193 ± 0.007 |
| S40T | 4.99 ± 0.09 | 3.94 ± 0.16 | 1.27 ± 0.03 |
| N63T | 5.38 ± 0.12 | 4.01 ± 0.10 | 1.343 ± 0.005 |
| N63T/V57I | 5.40 ± 0.16 | 3.87 ± 0.12 | 1.40 ± 0.03 |
| S40T/V57I | 4.23 ± 0.07 | 3.66 ± 0.22 | 1.16 ± 0.06 |
| S40T/N63T | 4.50 ± 0.12 | 3.85 ± 0.07 | 1.17 ± 0.01 |
| S40T/V57I/N63T | 4.197 ± 0.004 | 3.49 ± 0.05 | 1.20 ± 0.02 |
a
Parameters from Duncan et al. 2009 [31]