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Dapagliflozin protect HK-2 cells from hypoxia injury. A, Flow cytometric detection of annexin-V/PI staining. PI+ cells are necrotic and late apoptotic, PI−/AV+ cells are early apoptotic, and PI−/AV− cells are normal. B, Quantitative analysis of apoptotic and necrotic cells by flow cytometry. *P< 0.05 versus the hypoxia 1% O2 24 hours group. #P< 0.05 versus the control group. n = 5 in each group. There are 3 replicates in each group for cellular experiments.
