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The effect HE4 on the expression of HIF-1α, NF-κB, and cleaved caspase-3 in HK-2 cells under hypoxic conditions. A, Western blot analysis of the expression of HIF-1α, HE4, and NF-κB after HK-2 cells were transfected with either pSilencer vector containing a HE4-targeting sequence or control vector. B, Representative quantitative analysis (n = 5 in each group). *P < 0.05 compared with the pSilencer-HE4 group. There are 3 replicates in each group for cellular experiments.
