TABLE 2.

Primers used for PCR and sequencing

Primer designation	Gene	Nucleotide sequence (5′→3′)	Position (nt)	Type of analysis	Cycle temp (°C), time (min)
HS1a	16S rRNA	AAC GAT GAA GCT TCT AGC TTG CTA G	65–89	PCR and sequencing	94, 1
					60, 1.5
HS2a		GTG CTT ATT CGT TAG ATA CCG TCA T	464–439		72, 1
FH1b	16S rRNA	CGC TAA AGG ATT GGT CTA TGT CCT ATC	173–199	PCR and sequencing	94, 1
					65, 1
FH2b		TAT TCA CCG CAA CAT RGC TGA TTT G	1306–1282		72, 1
ISb	16S rRNA	TTA CCT AGG CTT GAC ATT GAA	909–929	Sequencing
IRb		TTC AAT GTC AAG CCT AGG TAA	929–909
AL1c	glmM	GGA TAA GCT TTT AGG GGT GTT AGG GG	1289–1314	PCR	94, 1
					56, 1
AL2c		GCT TAC TTT CTA ACA CTA ACG CGC	1584–1561		72, 1

^aPrimer numbering is based upon 16S rRNA gene sequence of H. pylori MC123 (GenBank accession no. UO1328). 

^bPrimer sequence and numbering are based upon 16S rRNA gene sequence of G. hominis G1A1 (GenBank accession no. L10079). 

^cPrimer sequence and numbering are based upon glmM gene sequence of H. pylori taxon 210 (GenBank accession no. M60398).