Fig 4. Lineage designations of whole genome and N gene sequences from the RABV Cosmopolitan clade.

A: Maximum likelihood tree showing the lineage positions, rooted using an outgroup of 10 Asian SEA1b sequences, with scale indicating substitutions per site per year, and hierarchical relationships with dashed lines indicating 5 lineage iterations (n = 2608 total sequences for tree and sunburst plot, combing both WGS and N gene sequences). B: Progression of classification definition from 22 previously defined subclades (left) to 52 lineages using only whole genome sequences (middle) to 147 lineages adding N gene sequences, with lineages seen at whole genome level in blue and additional lineages only seen at N gene level in red (right).