Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 26;12(12):2167–2181. doi: 10.1021/acschemneuro.1c00169

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

[³⁵S]GTPγS autoradiography in rat brain coronal sections at two different levels from Bregma, (A) including NBM and (B) including dorsal hippocampus, obtained from aCSF, left (n = 9), and SAP-treated rats, right (n = 11), that show representative autoradiograms of [³⁵S]GTPγS binding evoked by carbachol (100 μM). This assay is specific to G_i/o coupled receptors; therefore we are measuring the activity mediated by M₂/M₄ mAChR. The graphs show the mean ± SEM of each group in the different analyzed areas. NBM: nucleus basalis magnocellularis. D gyrus (granular): granular dentate gyrus. CA3: CA3 region of hippocampus. S sensory: somatosensory cortex. [¹⁴C]-Microscales were used as standards in nCi/g t.e. Scale bar: 5 mm.