TABLE 1.

Summary of mutations generated for each sgRNA.

Name for sgRNA	gRNA-PAMa	No. hairy roots sampled	No. hairy roots mutation detected	% mutations
SP1	5′-AGCTTTGGAGCTAAG GGTGATGG-3′	20	15	75
SP2	5′-TGTACTTAGCTTTGGA GCTAAGG-3′	19	3	15.8
SP3	5′-ACTGAAATAGAAGATC TGCATGG-3′	18	4	22.2
SP4	5′-GGAGGAACTATCAATG GCAATGG-3′	16	6	37.5
SP5	5’-TTTCAGACTACAAAGA TAGAAGG-3′	18	0	0

^aThe PAM sequences are highlighted in bold.