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. 2022 May 26;2022:8890434. doi: 10.1155/2022/8890434

Figure 4.

Figure 4

BMSC-derived exosomal miR-181a regulates FOXP3 via SIRT1-mediated acetylation. (a) The binding site of miR-181a and SIRT1 predicted by StarBase. (b) The interaction of SIRT1 and miR-181a determined by a dual-luciferase reporter assay. ∗P < 0.05. (c) Expression of SIRT1 and FOXP3 in CD4+ T cells treated with BMSC-derived exosomes detected by Western blot. ∗P < 0.05. (d) Expression of SIRT1 and FOXP3 in CD4+ T cells transfected with NC or miR-181a inhibitor BMSC-derived exosomes detected by Western blot. ∗P < 0.05. (e) FOXP3 acetylation in CD4+ T cells treated with BMSC-derived exosomes detected by immunoprecipitation. ∗P < 0.05. (f) FOXP3 acetylation in CD4+ T cells with CD4+ T cells transfected with NC or miR-181a inhibitor and BMSC-derived exosomes detected by immunoprecipitation. ∗P < 0.05. Data are the mean ± SD (n = 3 biological replicates).