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. 2022 Apr 2;36(6):1541–1549. doi: 10.1038/s41375-022-01554-9

Fig. 4. c-MYB ablation by WFA is associated with induction of the unfolded protein response in AML cells.

Fig. 4

a–c GSEA of gene sets associated with a ribosome biogenesis, b unfolded protein response and c HSF1 activation in gene expression changes in THP1 cells following treatment with DMSO or 1 μM WFA for 6 hours. d, e Example (left panel) and quantification (right panel) of c-MYB, phospho-eIF2S1 and total eIF2S1 expression in THP1 cells treated for 6 h with the indicated concentrations of d WFA and e thapsigargin. Data are normalized to actin loading control and to DMSO treated controls. Bars and error bars are means and SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant, one sample t-test. f OPP incorporation in THP1 cells treated with 25 µg/ml CHX or 1 µM WFA for 4 h, normalized to DMSO treated control. Bars and error bars are means and SD of three independent experiments. ***P < 0.001; n.s. not significant, one sample t-test.