FIG. 3.
Induction of expression of lateral flagellin in A. lipoferum 4B (A) and 4VI (B) cells by antibody agglutination of the polar or lateral flagella. Polar flagellin (As-Fla) or lateral flagellin (As-Laf) polyclonal antibodies diluted in ultrapure water were added to exponential-phase-grown cultures of A. lipoferum 4B and 4VI, respectively. Whole-cell protein extracts were used for immunodetection of lateral flagellin. Equal amounts of total protein (260 μg) were loaded in each sample on the gel. Incubation with preimmune antisera led to the same result (data not shown) as that in the control experiment, in which cells were incubated with ultrapure water (panels A and B, lanes 1). Antibodies (As-Fla [A] or As-Laf [B]) were added to final dilutions of 100 (lane 2), 10−1 (lane 3), 10−2 (lane 4), 10−3 (lane 5), 10−4 (lane 6), and 10−5 (lane 7).