Fig. 2. Ablation of ATOM69 stimulates recruitment of proteins to the mitochondrion.

a Whole cellular extracts of uninduced and induced ATOM69-RNAi cells were subjected to stable isotope labelling by amino acids in cell culture (SILAC)-based quantitative mass spectrometry (MS). Top, volcano plot depicting mitochondrial proteins (red dots) as defined in⁵². The model substrates FtsH and cytochrome oxidase subunit IV (COXIV) are highlighted. Bottom, volcano plot depicting other proteins (grey dots). Highlighted in blue are the four putative non-mitochondrial candidate proteins that were further studied. b Ratio plots depicting changes in protein levels in crude mitochondrial fractions (Mito) versus whole cell extracts of ATOM69 RNAi experiments. Proteins are labelled as in a.