Fig. 5. SILAC-IP of TbUbL1-myc from whole cell extracts in the presence and absence of ATOM69.

Stable isotope labelling by amino acids in cell culture-immunoprecipitation (SILAC-IP) experiments of a wildtype cells (TbUbL1-myc +Tet) or b an induced atypical translocase of the outer membrane 69 (ATOM69) RNAi cell line (TbUbL1-myc/ATOM69-RNAi +Tet) both expressing tetracycline inducible ubiquitin-like protein 1 (TbUbL1). Proteins shown were quantified in 3/3 independent replicates. p values were determined following the rank sum method⁸³. Highlighted are the bait TbUbL1-myc (red), ubiquitin (yellow), two other proteins that are enriched in both cell lines (blue), and nuclear proteins (green). Annotation of nuclear proteins is based on Tryptag⁸⁷ or the nuclear proteome⁸⁸. The horizontal dashed lines indicate a false discovery rate of 5%.