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. 2022 Jun 2;12:9224. doi: 10.1038/s41598-022-12986-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Simultaneous Porsolt forced swim test of two mice on day 1. (a) Schematic diagram of the experiment. Graphs showing the total number of immobility episodes (b) and the immobility episodes during each 1-min period (c). Graphs showing the total time spent immobile (d) and the proportion of time spent immobile during each 1-min period (e). All data are presented as box plots (b, d) or means ± standard errors (c, e). Statistical significance is represented by asterisks: *p < 0.05, ⁺p < 0.1. The p values were calculated using one-way analysis of variance (ANOVA) in (b) and (d) and two-way repeated-measures ANOVA in (c) and (e). SEM: standard error of the mean. individual: n = 10, opaque partition: n = 10, no partition: n = 10. Respective mean and SEM are listed in Table 2.