Fig. 3. The localization of wild-type and mutant PHEX, secPHEX, and FGF23, as detected using immunofluorescence in HEK293T cells co-transfected with PHEX, secPHEX, and FGF23.

Wild-type PHEX, secPHEX, and FGF23 proteins are distributed in the cell membrane or endoplasmic reticulum, while the mutant is located in the nuclear membrane (possibly endoplasmic reticulum) and cytoplasm. In order to clearly show the positional correlation between co-transfected FGF23 and PHEX expressed in cells, the figures were overexposed. WT wild type, mut mutation type, sec secretory type. Scale bar, 10 μm.