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. 2022 Jun 2;13(6):517. doi: 10.1038/s41419-022-04913-7

Fig. 3. Knockdown of circSCAF8 can suppress the ability of proliferation, invasion, and migration of PCa cells.

Fig. 3

A The knockdown efficiency of the two shRNAs that target circSCAF8 in PC3 and 22RV1 cells. B The expression level of circSCAF8 after transfected with circSCAF8 expression vectors in PC3 and 22RV1 cells. C qRT-PCR analysis of SCAF8 mRNA expression after treatment with sh1-circSCAF8 and circSCAF8 expression vectors. D–F The proliferation ability of PC3 and 22RV1 cells after knockdown of circSCAF8 was measured by CCK-8 D, colony formation (E), and EDU F assays. G The invasion ability of PC3 and 22RV1 cells was determined using a transwell invasion assay. H, I Transwell migration and wound healing assays were implemented to assess the migration ability of PC3 and 22RV1 cells after the knockdown of circSCAF8. J Cell cycle analysis revealed that PCa cells were blocked at the G1 phase after knockdown of circSCAF8. Error bars represent the SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns nonsignificant, PCa prostate cancer, EDU 5-ethynyl2′deoxyuridine.