Table 3.
Summary of the effect of different adjuvants on homo- (H5) and hetero- (H1) subtypic cross-reactivity and polarization of the Th1 vs Th2 response.
| Agonist | Without AddaVax | With AddaVax | ||||||
|---|---|---|---|---|---|---|---|---|
| IgG H5 | IgG H1 | IgG1 IL-4 |
IgG2c IFN-γ |
IgG H5 | IgG H1 | IgG1 IL-4 |
IgG2c IFN-γ |
|
| TLR2/6_4_7 | ++ | − |
++ ++ |
− − |
++ | + |
++++ + |
− − |
| TLR2/6, 4, 7 | − | − |
− +++ |
− − |
+++ | ++ |
+++++ ++ |
+ + |
| TLR2/6 | + | − |
+ +++ |
− − |
++ | + |
++++ + |
− − |
| TLR4 (PID) | − | − |
− + |
− − |
++++ | ++ |
+++++ ++++ |
+ − |
| TLR7 | − | − |
++ − |
− + |
+++ | ++ |
+++ + |
+ − |
| TLR9 | − | − |
− − |
− + |
++++ | ++ |
+++ + |
++ ++++ |
| TLR 4 (MPLA) | +++ | + |
+ − |
++ +++ |
+++ | ++ |
+++ ++ |
++ ++++ |
|
TLR 9, 4 (CpG, MPLA) |
+++ | ++ |
− − |
+++++ ++++ |
++++ | +++ |
+ ++++ |
++++ ++++ |
| None | − | − |
− ++ |
− − |
+++ | ++ |
++++ ++ |
+++ + |
Data for homo- and heterosubtypic cross-reactivity from Fig. 1, data for Th1 and Th2 response from Fig. 3. Key: For IgG, IgG1 and IgG2c, median signal intensities −, < 2000; +, 2000–9999; ++, 10,000–19,999; +++, 20,000–29,999; ++++, 30,000–39,999; +++++, > 40,000. For numbers of IL-4 and IFN-γ spot forming cells (SFCs) per 5 × 105 splenocytes: −, < 10; +, 10–24; ++, 25–49; +++, 50–75; ++++, > 75.