Fig. 6. Recognition of YscXY by YscVC depends on the C-terminus of YscX as well as SD3 and SD4 of YscVC.
a Coomassie-stained SDS-PAGE of MBP pull-downs. N- and C-terminally truncated MBP-YscX constructs were co-expressed with His6-YscY and YscVC. Lysates were loaded onto amylose resin and the washed matrix loaded onto the gel. The pull-downs generated consistent results over three replications. Source data are provided as a Source Data file. b Analytical size exclusion chromatography with 10 µM YscVC, YscVSD12 (both blue), YscX32:YscY (black), or mixtures (red) of the proteins. Absorbance at 280 nm shows a shift in retention volume for a mixture of YscVC with YscX32:YscY, but not YscVSD12 with YscX32:YscY. Chromatographies involving YscVSD12 or YscVC were performed twice or four times, respectively, with consistent results. A SDS-PAGE and Western blot analyzing contents of gel filtration peaks are shown in Supplementary Fig. 8.