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. 2022 Jun 2;9:264. doi: 10.1038/s41597-022-01353-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Synchrotron-based hierarchical phase-contrast tomography (HiP-CT) at multiple lengthscales and their associated data acquisition and image reconstruction pipelines. The full-field tomography data at 25.08 μm voxel size are processed with pipeline (a). The local tomography data for volumes of interest (VOIs) at 6.05–6.5 μm (level 1) and 2.45–2.5 μm (level 2) voxel size are processed with pipeline (b). (c) A schematic illustrating the relationships of the various cylindrical volumes imaged with HiP-CT. The triple arrows in the pipeline before merging the subscans indicate that the same procedure is carried out on each subscan.