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. 2022 Jun 2;13:3093. doi: 10.1038/s41467-022-30610-2

Fig. 3. MUC1 avoids positively-curved membranes and prefers negatively-curved ones induced by membrane-sculpturing proteins.

Fig. 3

A, B Schematic illustrations of A IRSp53-induced negative membrane curvature and B FBP17-induced positive membrane curvature. C, D Confocal images of U2OS cells transfected with either MUC1_0TR-GFP or MUC1_42TR-GFP and co-transfected with either C IRSp53-mCherry to induce membrane protrusions with negative curvature; or D mCherry-FBP17 to generate membrane invaginations with positive curvature. Scale bars represent 10 µm. E Colocalization analysis of MUC1s of four lengths and two mCherry-BAR-family proteins in U2OS cells shows that longer MUC1s with 21TRs and 42TRs are preferentially excluded from FBP17-induced membrane invaginations (see Supplementary Table 4A, B for the detailed statistics). F Colocalization analysis of MUC1 and two mCherry-BAR-family proteins in Hela cells shows a similar trend (see Supplementary Table 4C for the detailed statistics). All cells were cultured on flat surfaces. Welch’s t-tests (unpaired, two-tailed, not assuming equal variance) are applied for all statistical analyses in this figure. Error bars represent SEM. Arrows were drawn for guidance purpose.