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. 2022 Jun 2;11(1):31. doi: 10.1038/s41389-022-00404-8

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A–C USP53 mRNA and protein levels in Huh-7 and HCCLM3 cell lines in which USP53 overexpression plasmid was suitably transfected. D–G Representative images of the wound-healing assay showing migration distance of the control and USP53-overexpressing cells. Magnification 100x. Scale bar:100 μm. The wound-healing percentage was calculated by the distance changes between the two sides. H, I Representative images of the trans-well assay showing invasion capacity of control and USP53-overexpressing cells. Scale bar:100 μm. J, K Percentage of the proliferating cells in the indicated groups. There were 2000 cells per group initially. n = 6. L, M Representative images showing EdU uptake in the control and USP53-overexpressing Huh-7 and HCCLM3 cells. EdU was marked by red fluorescent dyes. DAPI which stained the nucleus showed the blue fluorescence. Magnification 400x. Scale bar:100 μm. N, O Number of colonies formed by the control and USP53-overexpressing Huh-7 and HCCLM3 cells. Scale bar: 10 mm. P–R Flow cytometry plots showing percentage of apoptotic control and USP53-overexpressing Huh-7 and HCCLM3 cells. The cells were marked by annexin V. S–U Flow cytometry plots showing the cell cycle distribution of control and USP53-overexpressing Huh-7 and HCCLM3 cells. All experiments were repeated three times. *p < 0.05; **p < 0.01; ***p < 0.001.